Effects of T2DM on postoperative outcome of patients with colorectal cancer: a study on the relationship between blood glucose control and survival rate.

To investigate the impact of type 2 diabetes (T2DM) on the prognosis of colorectal cancer (CRC). The data of 312 patients with CRC treated in the First Affiliated Hospital of Huzhou University from 2012 to 2018 were analyzed retrospectively. The patients were divided into a comorbidity group (n = 62) and a non-comorbidity group (n = 250) according to the presence of T2DM. The baseline data of the two groups were balanced by 1:2 propensity score matching (PSM). Kaplan-Meier analysis and Log-rank test were employed to compare the 5-year overall survival (OS) rates of patients. Cox regression model and inverse probability of treatment weighting (IPTW) were utilized to assess the influence of T2DM on 5-year OS of patients. Based on the results of Cox regression, a nomogram model of T2DM on 5-year OS of patients was constructed. A total of 62 patients in the comorbidity group and 124 patients in the non-comorbidity group were matched using PSM. The 5-year OS rate was lower in the comorbidity group than in the non-comorbidity group (82.23% VS 90.32%, P = 0.038). Subgroup analysis showed that the 5-year overall survival rate was higher in the good blood glucose control group than in the poor blood glucose control group (97.14% VS 62.96%, P<0.01). Multivariate Cox regression showed that the 5-year mortality risk in the comorbidity group was 2.641 times higher than that in the non-comorbidity group (P = 0.026). IPTW analysis showed that the 5-year risk of death in the comorbidity group was 2.458 times that of the non-comorbidity group (P = 0.019). The results showed that poor blood glucose control, BMI≥25 kg/m2, low differentiation, III/IV stage, and postoperative infection were independent factors affecting the 5-year overall survival rate of CRC patients (P<0.05). The ROC curve showed that the AUCs of the constructed model in predicting the 5-year OS in the training set and the testing set were 0.784 and 0.776, respectively. T2DM is identified as a risk factor for reduced 5-year survival among CRC patients, necessitating increased attention for this subgroup, particularly those with poor blood glucose control.

Association of myopia and astigmatism with postoperative ocular high order aberration after small incision lenticule extraction.

OBJECTIVE: To investigate the correlation between higher-order aberrations (HOA) after small incision lenticule extraction (SMILE) and the severity of myopia and astigmatism, along with the relevant factors. These findings will provide valuable insights for decreasing the occurrence of HOA after SMILE and enhancing visual quality. METHODS: A total of 75 patients (150 eyes) with myopia and astigmatism who underwent SMILE were categorized into four groups based on the severity of myopia and astigmatism: Myopia Group 1 (Group M1, spherical diopter ranged from -1.00 D to -4.00 D), Myopia Group 2 (Group M2, spherical diopter ranged from -4.10 D to -10.00 D), Astigmatism Group 1 (Group A1, cylindrical diopter ranged from 0 D to -1.00 D), and Astigmatism Group 2 (Group A2, cylindrical diopter ranged from -1.10 D to -3.00 D). A comprehensive assessment was performed to examine the association between HOA and various relevant factors, including a detailed analysis of the subgroups. RESULTS: Group M1 had significantly lower levels of total eye coma aberration (CA), corneal total HOA (tHOA), internal tHOA, and vertical CA ( Z 3 - 1 ) after SMILE than Group M2 (P < 0.05). Similarly, Group A1 had significantly lower levels of total eye tHOA, CA, trefoil aberration (TA), corneal tHOA, TA, and vertical TA ( Z 3 - 3 ) after SMILE than Group A2 (P < 0.05). Pearson correlation analysis indicated a statistically significant positive relationship between the severity of myopia/astigmatism and most HOA (P < 0.05). Subgroup evaluations demonstrated a notable increase in postoperative HOA associated with myopia and astigmatism in Groups M2 and A2 compared with the control group. Lenticule thickness, postoperative central corneal thickness (CCT), postoperative uncorrected distance visual acuity (UDVA), and postoperative corneal Km and Cyl were strongly correlated with most HOA. Age, eyes, and postoperative intraocular pressure (IOP) were only associated with specific HOA. CONCLUSION: HOA positively correlated with the severity of myopia and astigmatism after SMILE. However, this relationship was not linear. HOA after SMILE was influenced by various factors, and additional specialized investigations are required to establish its clinical importance.

Differences in ocular high order aberrations before and after small incision lenticule extraction for correction of myopia: a systematic review and meta-analysis.

Objective: To examine the causes and factors that lead to high order aberration (HOA) during the treatment of myopia using small incision lenticule extraction (SMILE), as well as the differences between SMILE and other corneal refractive surgeries through a systematic review and meta-analysis. Methods: A systematic search was conducted from January 2015 to February 2023 in Pubmed, Embase, Web of Science, and Google Scholar databases to gather relevant studies on SMILE and HOA. Studies meeting specific criteria were chosen, and clinical data was retrieved for analysis. Results: This meta-analysis resulted in the inclusion of 19 studies involving 1,503 eyes. Pooled results showed significant induction of total HOA (tHOA, d = -0.21, p < 0.001), spherical aberration (SA, d = -0.11, p < 0.001) and coma aberration (CA, d = -0.18, p < 0.001) after SMILE compared to pre-SMILE, while no significant change in trefoil aberration (TA) was observed (d = -0.00, p = 0.91). There was a significantly lower induction of tHOA after SMILE compared to femtosecond laser-assisted in situ keratomileusis (FS-LASIK, d = 0.04, p < 0.001), and no significant difference was observed compared to wavefront aberration-guided (WFG) refractive surgery (d = 0.00, p = 0.75). There was also a significant association between different levels of myopia and astigmatism, duration of follow-up, lenticule thickness, and preoperative central corneal thickness (CCT) on the induction of tHOA after SMILE (p < 0.05), while the higher preoperative myopia group (sphere > -5D), lower preoperative astigmatism group (cylinder ≤ -1D), larger lenticule thickness group (lenticule thickness > 100 µm), shorter follow-up group (follow-up 1 month postoperatively) and the thicker CCT group (CCT > 550 µm) brought a significant induction of tHOA compared to the opposite comparison group (p < 0.001). Conclusion: While SMILE can induce HOA significantly, it induces less HOA than FS-LASIK. Postoperative HOA following SMILE can be affected by factors such as myopia, astigmatism, lenticule thickness, CCT, and duration of follow-up. Future research should continue to explore techniques to decrease the induction of HOA by using this methodology. Systematic review registration: https://www.crd.york.ac.uk/prospero/.

A study on the effect of using the video teach-back method in continuous nursing care of stroke patients.

Objective: To explore the effect of a video teach-back method on continuous family nursing care of stroke patients. Methods: Stroke patients hospitalized in our hospital between March 2020 and March 2023 who met the inclusion criteria were randomly divided into an intervention group (n = 45), who received routine health education plus video teach-back training of caregivers, and a control group (n = 45), who received routine health education only. The effects on nursing-related variables were compared between the two groups. Results: Total scores representing the caring ability of caregivers in the intervention group increased significantly over time relative to baseline and were higher than those of the control group. Scores representing the care burden of caregivers in the intervention group decreased significantly over time and were lower than those of the control group. Conclusion: The teach-back method combined with video education improves the nursing ability of family caregivers and can improve the self-care ability of stroke patients.

Efficacy and safety of gut microbiota-based therapies in autoimmune and rheumatic diseases: a systematic review and meta-analysis of 80 randomized controlled trials.

BACKGROUND: Previous randomized controlled trials (RCTs) suggested that gut microbiota-based therapies may be effective in treating autoimmune diseases, but a systematic summary is lacking. METHODS: Pubmed, EMbase, Sinomed, and other databases were searched for RCTs related to the treatment of autoimmune diseases with probiotics from inception to June 2022. RevMan 5.4 software was used for meta-analysis after 2 investigators independently screened literature, extracted data, and assessed the risk of bias of included studies. RESULTS: A total of 80 RCTs and 14 types of autoimmune disease [celiac sprue, SLE, and lupus nephritis (LN), RA, juvenile idiopathic arthritis (JIA), spondyloarthritis, psoriasis, fibromyalgia syndrome, MS, systemic sclerosis, type 1 diabetes mellitus (T1DM), oral lichen planus (OLP), Crohn's disease, ulcerative colitis] were included. The results showed that gut microbiota-based therapies may improve the symptoms and/or inflammatory factor of celiac sprue, SLE and LN, JIA, psoriasis, PSS, MS, systemic sclerosis, Crohn's disease, and ulcerative colitis. However, gut microbiota-based therapies may not improve the symptoms and/or inflammatory factor of spondyloarthritis and RA. Gut microbiota-based therapies may relieve the pain of fibromyalgia syndrome, but the effect on fibromyalgia impact questionnaire score is not significant. Gut microbiota-based therapies may improve HbA1c in T1DM, but its effect on total insulin requirement does not seem to be significant. These RCTs showed that probiotics did not increase the incidence of adverse events. CONCLUSIONS: Gut microbiota-based therapies may improve several autoimmune diseases (celiac sprue, SLE and LN, JIA, psoriasis, fibromyalgia syndrome, PSS, MS, T1DM, Crohn's disease, and ulcerative colitis).

Changes of dry eye parameters after small incision lenticule extraction surgery in patients with different ocular surface disease index scores.

To evaluate the changes of dry eye parameters after small incision lenticule extraction (SMILE) surgery in patients with different ocular surface disease index (OSDI) scores. Prospective research. Participants were divided into two groups: Group A, OSDI < 13; and Group B, OSDI ≥ 13. The OSDI scores, tear meniscus height (TMH), first non-invasive tear film break-up time (NIBUT-First), and meibomian gland loss (MGL, %) were recorded at postoperative 1 -week and 1-month.113 eyes (57 patients) were enrolled, 70 eyes in Group A, and 43 eyes in Group B. In Group A, the OSDI scores significantly increased at 1-week and 1-month postoperative (all P < 0.001); the TMH, NIBUT-First and lipid layer grade significantly decreased at postoperative 1-week (P = 0.003, 0.005, 0.007, 0.004, respectively), but returned to preoperative level at 1-month postoperative. In Group B, only the lipid layer grade significantly decreased at postoperative 1-week (P < 0.05). Patients with different preoperative OSDI scores may experience different changes early after SMILE surgery. Patients with OSDI scores < 13 may experience more dramatic changes in dry eye symptoms which would resolve, while subjective complains could still exists at 1 month after surgery.

[Distribution Characteristics and Influencing Factors of Abundant and Rare Planktonic Microeukaryotes in Jinsha River].

Planktonic microeukaryotes are usually composed of a few abundant species and a large number of rare species, which play an important role in maintaining the health and stability of aquatic ecosystems. At present, little is known about the biogeographical distribution patterns of these two groups of microeukaryotes in large damming rivers. This study analyzed the distribution patterns of abundant and rare planktonic microeukaryotes and the dominant factors affecting their spatial distributions in the Jinsha River, one of the largest rivers in southwestern China that is strongly regulated by cascade dams. The results showed that the alpha diversity of planktonic microeukaryotes in the Jinsha River was higher in the cascade dam reach than that in the upstream natural reach, and the increase in alpha diversity of rare species was larger than that of rich species. There were significant differences in microbial community composition among different river sections, and the relative abundances of the dominant genera such as Vermamoeba shared by them were also significantly different between the two river sections. The results of distance decay analysis revealed that the geographic distribution patterns of rare and abundant taxa were jointly influenced by environmental heterogeneity and dispersal limitation, and the results of variance decomposition analysis and partial Mantel further indicated that dispersal limitation was the dominant ecological process. The results provided data support for the distribution and ecological response of microorganisms in rivers in the areas lacking data in Southwest China.

[Biogeographic Distribution Patterns and Ecological Mechanisms of Benthic Eukaryotic Microorganisms in Jinsha River].

Eukaryotic microbial communities play an important role in biogeochemical cycling and maintaining the health and stability of ecosystems. However, little is known about the geographical distribution patterns, key driving mechanisms, and interspecific interactions of eukaryotic microorganisms in large rivers. This study investigated the geographical distribution characteristics, influencing factors, and interspecific interactions of eukaryotic microorganisms in sediments from the Jinsha River, a cascade hydropower development river in southwest China. The results showed that the α-diversity indices of benthic eukaryotic microorganisms in the Jinsha River increased first and then decreased. The community structure and relative abundance of dominant genera of benthic eukaryotic microorganisms differed significantly among different reaches. The results of distance decay analysis showed that the community assembly of benthic eukaryotic microorganisms in the Jinsha River was influenced by environmental selection and dispersal limitation. The results of variance partitioning analysis and neutral model further demonstrated that the geographical distribution pattern was mainly driven by dispersal limitation. The co-occurrence network analysis revealed that the interspecific competition and network connectivity of the cascade dam reach were weaker than those of the upstream natural reach, indicating that the interactions of benthic eukaryotic microorganisms were stronger in the upstream natural reach. This study supplemented the lack of data on the diversity and geographical distribution of benthic eukaryotic microorganisms in the river of southwest China and provided data support for the ecological response of benthic eukaryotic microorganisms in the river of cascade hydropower development.

Insight into different adsorption behaviors of two fluoroquinolone antibiotics by sediment aggregation fractions.

Sediment, consisting of different aggregation fractions, is a hotspot site for transport and transformation of various pollutants including antibiotics. However, the fate of different antibiotics in aquatic sediments mediated by sediment aggregation fraction adsorption and the mechanism behinds are still unclear. In this study, we investigated the adsorption behavior of two fluoroquinolone antibiotics (ciprofloxacin and ofloxacin) on four aggregation fractions separated from the sediment of Taihu Lake, a typical lake contaminated by antibiotics in China. The results showed that the adsorption of ciprofloxacin and ofloxacin fitted the Freundlich model, irrespective of sediment aggregation size. The adsorption of ciprofloxacin and ofloxacin was depended on the size of sediment aggregation fractions, and the macroaggregation (> 200 µm) exhibited the strongest capacity, followed by large microaggregation (63-200 µm), medium microaggregation (20-63 µm), and small and primary microaggregation (< 20 µm). This fraction size-dependent effects of sediment aggregations on antibiotic adsorption might be closely related to the differences in their specific surface areas, organic matter contents, and surface functional groups. The adsorption of ciprofloxacin and ofloxacin by sediment aggregation fractions was characterized by a combination of chemical and physical adsorptions, with the former being the dominant process. Compared with ofloxacin, ciprofloxacin could be more rapidly and easily absorbed by four sediment aggregation fractions, and more readily complexed with carboxyl groups on macroaggregation surface. The adsorption of two antibiotics by extracellular polymeric substance showed that tryptophan and tyrosine protein-like, humic-like substance on the surface of sediment could bind to both antibiotics through a complexation reaction. The π-π electron donor-acceptor interaction and hydrogen bonds were responsible for the antibiotic adsorption by sediment aggregation.

A meta-analysis of visual outcomes and optical quality after small incision lenticule extraction versus implantable collamer lens for myopia.

OBJECTIVE: To evaluate possible differences in visual outcome and optical quality when small incision lenticule extraction (SMILE) versus implantable collamer lens (ICL) is used to correct myopia. METHODS: PubMed, EMBASE, and the Cochrane Central Register of Controlled Trials were searched up to August 2021 to identify relevant studies. The PRISMA guidelines were followed. Primary outcomes were postoperative efficacy, safety, and predictability at the end of follow-up. Secondary outcomes were changes in modulation transfer function cutoff frequency (MTF cut-off), objective scatter index (OSI) values, total higher-order aberrations (HOAs), spherical aberrations, and coma aberrations. RESULTS: Seven studies with a total of 1296 eyes (SMILE: 731 eyes; TECXL: 565 eyes) were included. The ICL group had a better MTF cut-off (P = 0.007) and less change in the proportion of HOAs (P < 0.001), spherical aberrations (P = 0.009), and coma (P = 0.006) than the SMILE group. For the other outcomes, there were no statistically significant differences. CONCLUSIONS: SMILE and ICL implantation are comparable in efficacy, safety, and predictability for correcting myopia. Larger studies with longer follow-up times are warranted to provide a better understanding of the benefits of SMILE and ICL implantation.

Age- and gender-related characteristics of astigmatism in a myopic population.

Purpose: To explore age- and gender-related differences of refractive and corneal astigmatism in myopic patients looking for refractive surgery. Design: A retrospective cross-sectional study. Materials and methods: The medical files of candidates looking for corneal refractive surgery between 2019 and 2021 were reviewed, demographic and refractive parameters including age, gender, refractive status, and corneal parameters were analyzed. Results: A total of 1,417 eyes of 1,417 patients (453 males and 964 females) were included. Males had thicker cornea than females, while females had steeper cornea than males, there was no gender-related difference in refractive and corneal astigmatism depending on patients' age. There was no difference in refractive astigmatism among different age group from 18 to 50 years, while corneal astigmatism had a shift from with-the rule (WTR) to against-the-rule (ATR) with increasing age. Age, central corneal thickness (CCT), sphere, refractive astigmatism (RA), and corneal curvature (Km) were correlated with corneal astigmatism (CA) (standardized coefficients of are 0.006, p = 0.011 for age, -0.001, p = 0.004 for CCT, and -0.027, p < 0.001 for sphere, 0.61, p < 0.001 for RA, -0.05, p < 0.001 for corneal curvature). Conclusion: Refractive astigmatism is stable until the age of 50 years in myopic patients looking for refractive surgery, while corneal astigmatism showed a shift from WTR to ATR with advancing age. Age, CCT, sphere, refractive astigmatism and corneal curvature (Km) were correlated with corneal astigmatism.

NudCD1 as a prognostic marker in colorectal cancer and its role in the upregulation of cellular spindle assembly checkpoint genes and LIS1 pathways.

OBJECTIVE: To investigate the role of NudCD1 in spindle assembly checkpoint regulation and in the prognosis of colorectal cancer.  METHODS: Immunohistochemical staining was used to detect in situ expression of NudCD1 in 100 colorectal cancer tissue samples. A chi-square test was used to analyse the correlation between the NudCD1 protein expression level of the cancer tissues and clinicopathological features. The Kaplan-Meier survival analysis was used to assess the correlation between the NudCD1 mRNA expression and the three-year survival of patients with colorectal cancer. The impact of NudCD1 on the development of colorectal cancer and the underlying molecular mechanisms were assessed by flow cytometry cell cycle and apoptosis assays after lentiviral overexpression of NudCD1 in two colorectal cancer cell lines. Quantitative real-time PCR was used to assess mRNA expression of the cellular spindle assembly checkpoint genes BUB1, BUBR1, MAD1, CDC20 and MPS1, as well as the downstream genes LIS1, DYNC1H1, and DYNLL1 in the NudC/LIS1/dynein pathway. RESULTS: Compared with normal intestinal tissue (8.00% with high expression), the expression of NudCD1 protein in colorectal cancer tissue was significantly higher (58.00% with high expression, P < 0.01). In addition, expression of NudCD1 significantly correlated with the degree of tumour differentiation and the TNM staging (P < 0.01), as well as the depth of invasion of the primary tumour and lymph node metastasis (P < 0.05). However, there was no correlation with gender, age, tumour site, gross type, tumour size or distant metastasis. The Kaplan-Meier survival analysis showed that patients with high NudCD1 expression in colorectal cancer tissues had a significantly shorter survival time than those with low expression of NudCD1 (P < 0.01). Compared with the transfection of the empty vector, colon cancer HT-29 cells with overexpressed NudCD1 had significantly increased mRNA levels of BUBR1, MPS1 and LIS1. The DNA synthesis phase (S phase) was significantly shorter in cells overexpressing NudCD1 than in the control group (43.83% ± 1.57%, P < 0.05), while there was no difference in apoptosis in the two groups. CONCLUSION: NudCD1 can serve as a valuable prognostic marker for colorectal cancer. It may be involved in the regulation of spindle-assembly checkpoint-gene expression and the LIS1 pathway of colorectal cancer cells.

The Biocomplex Assembled from Antigen Peptide and Toll-like Receptor Agonist Improved the Immunity against Pancreatic Adenocarcinoma In Vivo.

Purpose: One of the biggest challenges in cancer immunotherapy is generating robust cancer-specific immunity. This work describes using a biocomplex assembled from a toll-like receptor agonist CpG oligodeoxynucleotide 1826 (CpG) and a pancreatic cancer antigen peptide mesothelin for tuning pancreatic tumor immunity. Methods: This biocomplex was assembled via electrostatic interactions and characterized in size, morphology, zeta potential, and cargo loading. The effect of biocomplex on cell viability and activation of DCs and macrophages were measured by flow cytometry. The production of cytokines (GM-CSF, TNF, and IL-6) was evaluated by using ELISA kits. The effect of biocomplex on tumor cell proliferation was also evaluated by in vivo tumor model. Result: We can modulate the surface charge of the biocomplex by simply varying the ratios of the two components. In cell models, this biocomplex did not impact cell viability in the antigen-presenting cell (i.e., dendritic cell and macrophage)-directed immunity. Moreover, this biocomplex regulated the secretion of tumor-related cytokines (i.e., GM-CSF, TNF, and IL-6) and promoted the activation of immune cell surface markers (i.e., CD80+, CD86+, and CD40+). In the mouse model, the biocomplex inhibited the tumor burden effectively and promoted the production of effector cytokines. Conclusion: The present studies showed that the biocomplex with antigen peptide and toll-like receptor agonist was able to potentiate the antitumor immunity in vivo. This study will help understanding of immunity in pancreatic cancer and developing new immune therapeutic strategies for pancreatic adenocarcinoma.

Traditional Uses, Phytochemistry, Pharmacology and Toxicology of Rhizoma phragmitis: A Narrative Review.

Rhizoma phragmitis is a common Chinese herbal medicine whose effects are defined as 'clearing heat and fire, promoting fluid production to quench thirst, eliminating irritability, stopping vomiting, and disinhibiting urine'. During the Novel Coronavirus epidemic in 2020, the Weijing Decoction and Wuye Lugen Decoction, with Rhizoma phragmitis as the main herbal component, were included in The Pneumonia Treatment Protocol for Novel Coronavirus Infection (Trial Version 5) due to remarkable antiviral effects. Modern pharmacological studies have shown that Rhizoma phragmitis has antiviral, antioxidative, anti-inflammatory, analgesic, and hypoglycemic functions, lowers blood lipids and protects the liver and kidney. This review aims to provide a systematic summary of the botany, traditional applications, phytochemistry, pharmacology and toxicology of Rhizoma phragmitis.

Insight into microbial degradation of hexabromocyclododecane (HBCD) in lake sediments under different hydrodynamic conditions.

Hexabromocyclododecane (HBCD), an emerging persistent organic pollutant, has been widely detected in aquatic ecosystems with various hydrodynamic conditions, however, the effects of hydrodynamic changes on microbial degradation of HBCD in aquatic sediment remains unclear. Here, we conducted an annular flume experiment to characterize variation in HBCD removal from contaminated sediment under three hydrodynamic conditions with different flow velocities, as well as clarify the underlying microbial mechanisms. We detected significant HBCD removal and bromine ion generation in all contaminated sediments, and microbial reduction debromination was an important process for HBCD removal. At the end of the 49-day experiment, both HBCD removal percentage and the bromine ion concentration were significantly lower under dynamic water condition with higher sediment redox potential, compared with static water conditions. The dynamic water conditions resulted a relatively high sediment redox potential and decreased the iron reduction rate and the abundance of organohalide-respiring bacteria (OHRB) in the genera Geobatcer, Dehalogenimonas, Dehalobacter, and Dehalococcoide, which reduced the microbial degradation of HBCD in contaminated sediments. The community composition of both total bacteria and OHRB also differed significantly among hydrodynamic conditions. Some bacterial groups with HBCD degradation abilities such as Pseudomonas and Sulfuricurvum were less abundant under dynamic water conditions, and the HBCD degradation efficiencies were lower. These findings enhance our understanding of the bioremediation potential of HBCD-contaminated sediments in different hydrodynamic areas.

How dam construction affects the activity of alkaline phosphatases in reservoir sediments: A study of two highly regulated rivers.

Dam construction causes phosphorus (P) accumulation in reservoir sediments and significantly affects the generation of available P. However, the effect of dam construction on the activity of sediment alkaline phosphatase (ALP), which is encoded by the bacterial phoD gene and participates in P mineralization, in river sediments remains unclear. Here, we investigated the ALP activities in 78 sediment samples collected from the cascade reservoir regions located in the Lancang River and the Jinsha River, two highly regulated rivers in southwestern China. The abundance and community composition of phoD-harboring bacteria were determined based on the phoD gene using quantitative real-time PCR and MiSeq sequencing, respectively. Comparison of control and affected sites indicated that dam construction significantly increased sediment ALP activity in both rivers. The abundances of phoD-harboring bacteria increased and their community compositions varied in response to dam construction; the relative abundances of the dominant genera Methylobacterium and Bradyrhizobium were particularly higher in affected site than control site. Co-occurrence network analyses revealed much higher network connectivity and relative abundances of keystone species in affected sites. Some microbial factors including phoD-harboring bacterial abundances, network clustering coefficients, and relative abundance of keystone species were positively correlated with ALP activity. The relative abundance of keystone species was identified as the most important microbial factor contributing to variation in ALP activity based on structural equation modeling analysis. These findings enhance our understanding of how dam construction affects the functions of phoD-harboring bacteria and their role in the P biogeochemical cycle in highly regulated rivers.

Design, synthesis and antibacterial activity evaluation of pleuromutilin derivatives according to twin drug theory.

A series of novel pleuromutilin derivatives were designed and synthesized based on the twin drugs theory. Piperazinyl and thioether were used as the linkage to connect the pleuromutilin nuclear and sulfonamide to improve the biological activity and water solubility of derivatives. The in vitro antibacterial activities against drug-sensitive and drug-resistance bacteria were evaluated by agar dilution method. Most of the 25 compounds displayed excellent antibacterial activities against drug-sensitive bacteria, particularly, five compounds (9, 10, 11, 14a and 14b) exerted the excellent antibacterial activities against drug-resistance bacteria.

Efficacy of small-incision lenticule extraction surgery in high astigmatism: A meta-analysis.

Purpose: This study aimed to evaluate the efficacy of small-incision lenticule extraction (SMILE) in improving vision and visual quality and correcting astigmatism for the treatment of high astigmatism. Methods: Eligible studies and studies comparing the efficacy of SMILE with femtosecond laser-assisted laser in situ keratomileusis (FS-LASIK) or transepithelial photorefractive keratectomy (T-PRK) for high astigmatism (≥2.00 D) were identified in PubMed, Embase, and the Cochrane Central Register of Controlled Trials, searched from their inception to 29 May 2022. The references of all searched studies were checked as supplements. The risk of bias was evaluated for each eligible study. The literature was screened according to the inclusion and exclusion criteria, and relative data were extracted. Data were extracted and analyzed by ReviewManager 5.4. The primary outcome was post-operative uncorrected distance visual acuity (UDVA). The secondary outcomes included corneal aberrations and vector parameters. The weight mean difference (WMD) and their 95% confidence intervals (95% CI) were used to assess the strength of the association. Results: A total of six studies including 380 astigmatism eyes were involved, with 211 high-astigmatism eyes and 31 low-astigmatism eyes undergoing SMILE surgery, 94 high-astigmatism eyes undergoing FS-LASIK surgery, and 44 high-astigmatism eyes undergoing T-PRK surgery. Compared with non-SMILE, SMILE induced more astigmatism (weighted mean difference [WMD] = -0.07, 95% CI [-0.12 to -0.02], P = 0.005) and fewer sphere aberrations (WMD = -0.12, 95% CI [-0.17 to -0.08], P < 0.00001). The post-operative UDVA, sphere, spherical equivalent (SE), and higher order aberrations in different surgeries were likewise equivalent. The difference vector and index of success were significantly higher, and the surgically induced astigmatism vector, correction index, and magnitude of error were significantly lower in SMILE. Conclusion: This meta-analysis suggests that SMILE, FS-LASIK, and T-PRK show excellent efficacy, predictability, and safety for myopia. SMILE exhibited less astigmatism refraction predictability and less surgically induced spherical aberrations. There may be more under-correction in SMILE. More randomized, prospective, and large sample-sized studies are needed to confirm these conclusions in the long term.

Comprehensive transcriptome analysis of colorectal cancer risk of hyperglycemia in humans.

BACKGROUND: Epidemiological studies have found that hyperglycemia, is an independent risk factor for colorectal cancer (CRC), increasing colon cancer incidence and affecting the recurrence, metastasis, and prognosis in colon cancer patients. However, the intercorrelation between hyperglycemia and CRC risk is still unknown, In the present study, we sought to determine whether gene markers, which act in CRC with hyperglycemia, are silenced in CRC without hyperglycemia. METHODS: In order to characterize the mechanism of functional genes associated with CRC with hyperglycemia, A total 24 CRC and matched controls were sequenced. Through bioinformatics analysis includes differential expression analysis, functional enrichment, new isoform prediction and alternative splicing event identification to found biomarker genes related to CRC development. RESULTS: CRC patients with hyperglycemia were compared with patients without hyperglycemia, and we found that 21 genes were upregulated and 27 were downregulated. Further study showed that these genes are possibly of key genes involved in CRC development with hyperglycemia, such as mannan-binding lectin-associated serine protease 3 (MASP3), which has an immunological role in the activation of the complement system. Based on our comprehensive analysis, a cis-regulatory network for hyperglycemic CRC was reconstructed. CONCLUSIONS: Protein-protein interactions revealed the mechanisms of molecules involved in the interaction of hyperglycemia and cancer development. Our results provide further information on the metabolic pathway interaction with cancer pathways and elucidated the mechanisms of hyperglycemic factors function in cancer development from a transcriptomic perspective.